Ligand trap useful in immunoassays of biotin or free biotin cont

Details Ligand trap useful in immunoassays of biotin or free biotin cont Ligand trap useful in immunoassays of biotin or free biotin cont

1990-05-09

1993-05-18

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 492, 435962, 436 53, 436512, 436535, 436175, C12Q 100, G01N 33544, G01N 33543, G01N 33566

Patent

active

052120635

ABSTRACT:
The present invention provides a process for the detection of analytes in body fluids containing free biotin by immunoassay with the use of biotin conjugates for the labelling, immobilization or complexing of immunological reagents, wherein the biotin present in free form is removed from the immunological reaction by incubating the sample solution with polymer particles consisting of a core and a covering which, as core, contain a polymer which has a plurality of binding sites for biotin and, as covering, at least one layer of protein, peptide, carbohydrate or co-polymer of carbohydrate and amino acids, the layer thickness of the covering being so adjusted that the coating is permeable for the free biotin but not for the biotin conjugate.

REFERENCES:
patent: 3951748 (1976-04-01), Devlin et al.
patent: 4282287 (1981-08-01), Giese
patent: 4550075 (1985-10-01), Bacquet et al.
patent: 4680274 (1987-07-01), Sakai et al.
patent: 4752638 (1988-06-01), Nowinski et al.
Bier et al. "Fundamentals of Immunology" 2nd Ed. 1986 Springer Verlag, pp. 85, 88.

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