Method for solubilizing proteins in organic solvents

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...

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530402, 530422, C12N 996

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active

056935161

ABSTRACT:
The present invention relates to a method for producing a protein composition soluble in organic solvents, comprising mixing a protein of interest with a surfactant and a water immiscible organic solvent in amounts and under conditions conducive to the formation of a reverse micelle solution, and evaporating the resulting reverse micelle solution to dryness.

REFERENCES:
Paradkar et al., J. Am. Chem. Soc., vol. 116, No. 11, pp. 5009-5010, 1994.
Basheer et al., Biotechnology & Bioenginnering, vol. 45, pp. 187-195, 1995.
Okahat et al., The Chem. Society of Japan, vol. 65, pp. 2411-2420, 1992.
Bromberg et al., Applied Biological Sciences, vol. 92, pp. 1262-1266, 1995.
Bromberg et al., Applied Biological Sciences, vol. 91, pp. 143-147, 1994.

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