Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1998-02-26
1999-09-21
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435183, 435194, 43525231, 435232, 233199, 233205, 233195, 233209, C12P 2106, C12N 900, C12N 120, C12N 988
Patent
active
059553103
ABSTRACT:
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to Bacillus cells for producing a polypeptide comprising a nucleic acid construct which comprises (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide.
REFERENCES:
Agaisse et al., Molecular Biology, 13 (1), pp. 97-107, (1994).
Hue et al., Journal of Bacteriology, vol. 177, No. 12, pp. 3465-3471, (1995).
Ichikawa et al., FEMS Microbiology Letters, 111, pp. 219-224, (1993).
Sloma Alan
Thomas Michael D.
Widner William
Monshipouri Maryam
Novo Nordisk Biotech Inc.
Starnes Robert L.
Wax Robert A.
Zelson Steve T.
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