Production of recombinant bone-inducing proteins

Details Production of recombinant bone-inducing proteins Production of recombinant bone-inducing proteins

1991-04-02

1994-06-07

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 703, 4352402, 4352403, C12P 2100, C12P 2102, C12N 502, C12N 510

Patent

active

053188985

ABSTRACT:
An improved process for producing recombinant bone-inducing protein of the BMP-2 family as described. A suitable mammalian host cell transformed with a DNA encoding the protein is cultured in an appropriate culture medium to which is added about 10 to 1000 .mu.g/ml dextran sulfate. The presence of dextran sulfate in the medium results in an increased yield of recombinant bone-inducing protein.

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Wang, et al. Recombinant Human Bone Morphogenetic Protein Induces Bone Formation, Proc. Natl. Acad. Sci. USA, vol. 87, pp. 2220-2224, Mar. 1990.
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Papkoff, Jackie, Mol and Cell. Biol. 9(8):3377-3384 (1989).
Baba, Masanori et al., Proc. Natl. Acad. Sci. U.S.A. 85:6132-6136 (1988).
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Hammonds, R. Glenn, Jr., et al., Molecular Endocrinology 5(1):149-155 (1991).

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