Composition, device and method of assaying for peroxidatively ac

Details Composition, device and method of assaying for peroxidatively ac Composition, device and method of assaying for peroxidatively ac

1990-01-30

1994-06-07

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

436 66, 436904, C12Q 128, G01N 3372

Patent

active

053188942

ABSTRACT:
A new and improved test device and method of determining the presence or concentration of a peroxidatively active substance, such as hemoglobin, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an indicator reagent composition capable of interacting with a peroxidatively active substance to produce a detectable or measurable response. In addition, a new and improved indicator reagent composition, comprising an indicator dye, such as a redox indicator, like a benzidine indicator; a hydroperoxide; a metal ion complex; and a phosphorous compound having the general structural formula ##STR1## wherein R.sub.1 or R.sub.2 is selected from the group consisting of hydrogen, an unsubstituted or a substituted alkyl moiety including from one to about twelve carbon atoms, an unsubstituted or a substituted aromatic moiety and a residue of an aliphatic or an aromatic polyhydric compound; and wherein X is 0, S or NH; such as glyceryl-2-phosphate or phenyl dihydrogen phosphate, is incorporated into a carrier matrix, or used in a wet phase assay, to provide a more accurate and trustworthy assay of a test sample for a peroxidatively active substance. The improved method and composition are especially useful in the assay of urine for occult blood.

REFERENCES:
patent: 4288541 (1981-09-01), Magers et al.
patent: 4310626 (1982-01-01), Burkhardt et al.
patent: 4615982 (1986-10-01), Lawrence

Affiliated with

Also associated with

Rating

Composition, device and method of assaying for peroxidatively ac does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Composition, device and method of assaying for peroxidatively ac, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Composition, device and method of assaying for peroxidatively ac will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-792248