Coating processes – Plant member or animal specimen coating
Patent
1993-04-05
1994-06-07
Beck, Shrive
Coating processes
Plant member or animal specimen coating
424 3, A01N 100
Patent
active
053187954
ABSTRACT:
In the field of staining methods for histology and cytology specimens, the improvements of the invention are three-fold. First included is a drying-clearing step whereby a specimen is cleared by drying. Thus, the use of hazardous chemical-clearing agents to remove washing-dehydrating solution prior to cover-slipping is eliminated. Second, in a staining method using spray staining equipment, the use of a low molarity, combination, bluing-washing solution which serves the dual purpose of a washing solution and a bluing reagent, eliminates the use of water in a staining procedure. Third, and also in a staining method using spray staining equipment, the use of a series of sprays of fresh, essentially anhydrous, washing-dehydrating solution to dehydrate a specimen replaces immersion in a series of washing-dehydrating solutions and thereby decreases the amount of chemical consumed and also avoids contamination of the reagent with water and specimen debris.
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J. S. Drijver, Acta Cytologica, vol. 27, No. 2, pp. 210-211 Mar. (1983).
Gibson John F.
Stokes Barry O.
Beck Shrive
Dudash Diana L.
Wescor, Inc.
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