Cryopreserved in vitro cultured epithelial tissue and method

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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43524023, 435240241, 435 1, 514 2, 514 21, 424574, A01N 102, A01N 3718, C12N 500, A61K 3700

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052984176

ABSTRACT:
A process for preserving cultured epidermal sheets by incubating the sheets at room temperature in media with a glycerol or dimethylsulfoxide cryopreservant for a predetermined period of time and then freezing the cultured sheets. The cooling gradient is characterized as having a lower initial rate of cooling followed by a higher rate of cooling.

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