Chemistry of carbon compounds – Miscellaneous organic carbon compounds
Patent
1980-02-13
1981-03-03
Schain, Howard E.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
260112R, 260112B, 2601127, 424 78, 424 85, 424 88, 424 91, A61K 3702, A61K 3726, C08L 7704, C08L 8900
Patent
active
042539960
ABSTRACT:
Techniques for preparing stable conjugates of protein-D-GL which can then be isolated in pure form are disclosed, using hen egg ovalbumin (OVA) as a prototype protein, and conjugation and purification methods are described which involve (1) application of a coupling method employing m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) as the coupling reagent, and (2) introduction of biotin moieties into D-GL molecules to allow application of avidinbiotin system for affinity chromatographic purification of conjugates. These methods are applicable to the preparation of D-GL conjugates of insulin and Ragweed Antigen E and are of general applicability.
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Schain Howard E.
Scripps Clinic and Research Foundation
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