Immunochemical conjugates: method and composition

Details Immunochemical conjugates: method and composition Immunochemical conjugates: method and composition

1980-02-11

1981-03-03

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

260112R, 424 12, 424 78, 424 85, 424 88, 424 91, C08L 7704, C08L 8900, A61K 3702, A61K 3726

Patent

active

042539951

ABSTRACT:
Techniques for preparing stable conjugates of protein-D-GL which can then be isolated in pure form are disclosed, using hen egg ovalbumin (OVA) as a prototype protein, and conjugation and purification methods are described which involve (1) application of a coupling method employing m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) as the coupling reagent, and (2) introduction of biotin moieties into D-GL molecules to allow application of avidinbiotin system for affinity chromatographic purification of conjugates. These methods are applicable to the preparation of D-GL conjugates of insulin and Ragweed Antigen E and are of general applicability.

REFERENCES:
patent: 3794630 (1974-02-01), Mullan et al.
patent: 4191668 (1980-03-01), Katz
Nossal et al., J. of Exp'l. Med., vol. 138 (1973), pp. 312-317.
Eshhar et al., J. Immunol., vol. 114, No. 2 (1975), pp. 872-876.
Bitter-Suermann et al., Chem. Abstracts, vol. 83, 129, 895 p, (1975).
Mosier et al., Chem. Abstracts, vol. 83: 191,237d (1975).
Eshhar et al., Chem. Abstracts, vol. 85: 121,584g (1976).

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