High specific activity nucleotide probes having target recogniti

Details High specific activity nucleotide probes having target recogniti High specific activity nucleotide probes having target recogniti

1993-10-25

1996-05-28

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 911, 435 912, 536 221, 536 231, 536 241, 536 2431, 536 2432, 536 2433, 536 253, 935 77, 935 78, C12Q 168, C07H 2104

Patent

active

055212980

ABSTRACT:
The probes of the invention are made up of a target recognition moiety and a signal generation moiety. In the first step of the preparatory method of the invention, the 5' or 3' terminus or both of the target recognition moiety is chemically altered to incorporate a reactive functionality which will allow chemical linkage of this target recognition moiety with one or both termini of the signal generation moiety of the probe. Suitable functionalities vary widely, but may, in general be described as those that allow chemical attachment of the two moieties either at the 5' or 3' end of the target recognition moiety. The two moieties should not detach from each other upon hybridization of the target moiety to the target analyte. Thus, when the probe is hybridized to target analyte, it will then carry with it, signal moiety, to label this complex, which label is then later detected.

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