Detection of specific sequences in nucleic acids

Details Detection of specific sequences in nucleic acids Detection of specific sequences in nucleic acids

1994-06-08

1996-05-28

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 915, 435 9152, 536 243, 935 17, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104

Patent

active

055210651

ABSTRACT:
A method of testing for the presence or absence of a target sequence in a mixture of single-stranded nucleic acid fragments is disclosed. The method involves reacting a mixture of single-stranded nucleic acid fragments with a first probe which is complementary to a first region of the target sequence, and with a second probe which is complementary to a second region of the target sequence, where the first and second target regions are contiguous with one another, under hybridization conditions in which the two probes become stably hybridized to their associated target regions. Following hybridization, any of the first and second probes hybridized to contiguous first and second target regions are ligated, and the sample is tested for the presence of expected probe ligation product. The presence of ligated product indicates that the target sequence is present in the sample.

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