Method of detecting sub-ppb levels of oligonucleotides in biolog

Details Method of detecting sub-ppb levels of oligonucleotides in biolog Method of detecting sub-ppb levels of oligonucleotides in biolog

1995-06-05

1997-06-10

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168

Patent

active

056374640

ABSTRACT:
Disclosed is a method of detecting a single-stranded target oligonucleotide in a biological fluid. In this method, a biological fluid sample to-be-tested is contacted with helper and primer oligonucleotides, thereby forming a labelled, double-stranded molecule if the sample contains an oligonucleotide which is complementary to the nucleotide sequences of the primer and the helper oligonucleotides. The primer so annealed to the target oligonucleotide is then ligated to the helper annealed to the target oligonucleotide. The presence of the ligation product is being indicative of the presence of the target oligonucleotide in the biological fluid.

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