Conjugation of ligand to immobilized protein in organic solvent

Details Conjugation of ligand to immobilized protein in organic solvent Conjugation of ligand to immobilized protein in organic solvent

1995-11-06

1998-10-06

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436526, 436529, 436530, 436533, 530807, 530811, 530814, 530815, G01N 33546, G01N 33552, G01N 33553

Patent

active

058175270

ABSTRACT:
A new method for immobilization of small molecules on solid supports via a macromolecular spacer has been developed. A protein or another macromolecule is first immobilized in an aqueous medium, and the solid support is than washed with an organic solvent. The small molecule is coupled in an organic medium, followed by organic medium washes. The new solid phases are useful for affinity purifications, immunoassays and other binding assays, and for selection of binders by panning procedures.

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