Spectrophotometric methods for assaying total mercaptans, reduce

Details Spectrophotometric methods for assaying total mercaptans, reduce Spectrophotometric methods for assaying total mercaptans, reduce

1996-08-12

1998-10-06

Soderquist, Arlen

Chemistry: analytical and immunological testing

Sulfur containing

Organic or sulfhydryl containing

430119, 430166, 422 61, 546152, 546179, 546180, G01N 3300

Patent

active

058175202

ABSTRACT:
According to the invention, the total mercaptans in an aqueous sample are assayed by means of the formation of a chromophoric substitution product between all the mercaptans present in the sample and a reagent corresponding to the general formula I: ##STR1## Reduced glutathione (GSH) is assayed in the form of a chromophoric thione formed specifically by alkalization from the substitution product formed above between GSH and the reagent.
The concentration of mercaptans distinct from GSH is obtained by subtraction of the concentrations obtained in the preceding assays.
Application to the assay of mercaptans and GSH, especially in the presence of other mercaptans, in particular in biological media.

REFERENCES:
patent: 2940974 (1960-06-01), Surrey
patent: 3002001 (1961-09-01), Surrey
patent: 3755332 (1973-08-01), Wasley et al.
patent: 3819374 (1974-06-01), Kemp
patent: 4058613 (1977-11-01), Abbruzzese et al.
patent: 4282219 (1981-08-01), Hannah
patent: 4431774 (1984-02-01), Felder-Schraner et al.
patent: 4678781 (1987-07-01), Jung
F. Tietze Anal. Biochem. 1969, 27, 502-522.
B. L. Ling et al, J. Chromatogr. 1991, 553, 433-439.
E. Sofic et al Fresenius J. Anal. Chem. 1991, 339, 258-260.
G. L. Ellman Arch. Biochem. Biophys. 1959, 82, 70-77.
J. Sedlak et al. Anal. Biochem. 1968, 25, 192-205.
N. P. Luthra et al. Anal. Biochem. 1981, 117, 94-102.
M. W. Fariss et al. Method. Enzymol. 1987, 143, 101-109.
G. C. Rice et al, Cancer Res. 1986, 46, 6105-6110.
E. M. Kosower et al, in "The Functions of Glutathione: Biochemical, Physiological, Toxicolgical & Clinical Aspects" A Larssen et al. ed, Raven Press; New York, 1983, pp. 243-250.
C. J. Holloway J. Chromatogr. 1987, 390, 101-110.
B. Mutus et al. Anal. Biochem. 1989, 177, 237-243.
O. W. Griffith Anal. Biochem. 1980, 106, 207-212.
P. J. Hissim et al. Anal. Biochem. 1976, 74, 214-226.
J. Martensson J. Chromatogr. 1987, 420, 152-157.
B. L. Ling et al. Anal. Chim. Acta 1989, 227, 203-209.
M. Inoue et al, J. Protein Chem. 1987, 6, 207-225.
C. J. Cooksey et al. J. Chem. Soc (B) 1968 1191-1197.
G. B. Barlin et al. J. Chem. Soc. Perkin Frans. II 1975, 298-302.
K. Matsumura J. Am. Chem. Soc. 1927, 49, 810-818.
F. Pirrone et al. Chem. Abstr. 1936, 30, 2194.
E. Moness et al. Chem. Abstr. 1936, 30, 5726.
R. F. Lutz et al. J. Am. Chem. Soc. 1946, 68, 1324-1325.
E. Pouterman et al. Experientia 1947, 3, 28.
R. H. Baker et al. J. Am. Chem. Soc. 1947, 69, 704-705.
W. H. Rueggeberg et al. J. Am. Chem. Soc. 1947, 69, 1222-1223.
A. R. Surrey et al. J. Am. Chem. Soc. 1959, 81, 2894-2897.
M. Tomita et al. Chem. Abstr, 1953, 47, 3314e.
T. Ueda et al. Chem. Abstr. 1955, 49, 10957a.
R. U. Schock J. Am. Chem. Soc. 1957, 79, 1670-1672.
H. Vogt et al. Chem. Abstr, 1958, 52, 16695a.
A. Rieche et al, Chem. Ber, 1959, 92, 2239-2252.
I. S. Ioffe et al. J. Gen. Chem. USSR 1960, 30,95-97.
G. Illuminati et al. J. Am. Chem. Soc. 1967, 89, 3521-3524.
J. W. Bunting et al. Can. J. Chem. 1974, 52, 962-974.
G. B. Barlin et al. J. Chem. Soc. Perkin Trans 2 1975 1267-1270.
W. O. Foye et al. J. Pharm. Sci. 1979, 68, 336-338.
E. Bald Talanta 1980, 27, 281-282.
C. R.o slashed.mming et al, Acta. Chem. Scand. B 1983, 37, 791-795.
P. J. Zavala et al. Talanta 1985, 32, 285-289.
Ling. B. Lin, Dewaele CL, Baeyens W.R.G. (1991) J. Chromatogr., 553(1-2), 433-9.

Affiliated with

Also associated with

Rating

Spectrophotometric methods for assaying total mercaptans, reduce does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Spectrophotometric methods for assaying total mercaptans, reduce, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Spectrophotometric methods for assaying total mercaptans, reduce will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-76340