Hirudin analogs, methods of manufacture thereof and anticoagulan

Details Hirudin analogs, methods of manufacture thereof and anticoagulan Hirudin analogs, methods of manufacture thereof and anticoagulan

1997-05-22

1999-10-26

Feisee, Lila

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 231, 4353201, 435325, 4352523, 4351723, 530324, 514 2, C07H 2104, C12N 1512, C12N 1563, C07K 1400

Patent

active

059726482

ABSTRACT:
Disclosed are hirudin analogs, a method of manufacture thereof, and anticoagulant compositions containing the same. Sequences Asp.sup.33 -Gly.sup.34 or Asp.sup.62 -Ala.sup.63 of naturally-occurring hirudins or their analogs were substituted as disclosed to suppress the formation of succinimide- or beta- forms, thereby yielding hirudin analogs with high stability, high anti-thrombin activity, and therapeutic potential as anticoagulants. Hirudin analogs having Val.sup.1 -Val.sup.2 of naturally-occurring hirudin substituted with Ile.sup.1 -Ile.sup.2 are preferred for their high antithrombiin activity. The invention also provides DNAs encoding the amino acid sequence of hirudin analogs, expression vectors, recombinant microogranisms, and a method of manufacturing hirudin analogs using recombinant microorganisms.

REFERENCES:
patent: 5118790 (1992-06-01), Winant et al.
patent: 5126134 (1992-06-01), Heim et al.
patent: 5192745 (1993-03-01), Krstenansky et al.
patent: 5192747 (1993-03-01), Krstenansky et al.
patent: 5204323 (1993-04-01), Findlay
Payne et al. (1991), "Positional Effects of Sulfation in Hirudin and Hirudin PA Related Anticoagulant Peptides," 34 J. Medicinal Chem. 1184-1187.
Broersma et al. (1991), "Antithrombotic Activity of a Novel C-Terminal Hirudin Analog in Experimental Animals," 65 Thrombosis & Haemostasis 377-381.
Lehman et al. (1993), "Expression, Purification and Characterization of Multigram Amounts of a Recombinant Hybrid HV1-HV2 Hirudin Variant Expressed in Saccharomyces cerevisiae," 4 Protein Expr. & Purif. 247-255.
Komatsu et al. (1993), "CX-397, A Novel Recombinant Hirudin Analog Having a Hybrid Sequence of Hirudin Variants-1 and -3,," 196 Biochem. & Biophys. Res. Comm. 773-779.

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