Computer-aided analysis system for sequencing by hybridization

Details Computer-aided analysis system for sequencing by hybridization Computer-aided analysis system for sequencing by hybridization

1998-10-22

1999-10-26

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

059726199

ABSTRACT:
The conditions under which oligonucleotide probes hybridize preferentially with entirely complementary and homologous nucleic acid targets are described. Using these hybridization conditions, overlapping oligonucleotide probes associate with a target nucleic acid. Following washes, positive hybridization signals are used to assemble the sequence of a given nucleic acid fragment. Representative target nucleic acids are applied as dots. Up to to 100,000 probes of the type (A,T,C,G)(A,T,C,G)N8(A,T,C,G) are used to determine sequence information by simultaneous hybridization with nucleic acid molecules bound to a filter. Additional hybridization conditions are provided that allow stringent hybridization of 6-10 nucleotide long oligomers which extends the utility of the invention. A computer process determines the information sequence of the target nucleic acid which can include targets with the complexity of mammalian genomes. Sequence generation can be obtained for a large complex mammalian genome in a single process.

REFERENCES:
patent: 4741043 (1988-04-01), Bacus
patent: 4777597 (1988-10-01), Shiraishi et al.
patent: 4802101 (1989-01-01), Hara
patent: 4811218 (1989-03-01), Hunkapiller et al.
patent: 4837733 (1989-06-01), Shiraishi et al.
patent: 4885696 (1989-12-01), Hara
patent: 4888695 (1989-12-01), Shiraishi et al.
patent: 4894786 (1990-01-01), Hara
patent: 4939667 (1990-07-01), Hara et al.
patent: 4941092 (1990-07-01), Hara et al.
patent: 4958281 (1990-09-01), Hara
patent: 4965725 (1990-10-01), Rutenberg
patent: 4972325 (1990-11-01), Hara
patent: 4982326 (1991-01-01), Kaneko
patent: 5002867 (1991-03-01), Macevicz
patent: 5260190 (1993-11-01), Shiraishi et al.
patent: 5270162 (1993-12-01), Shiraishi et al.
patent: 5297288 (1994-03-01), Hemminger et al.
patent: 5306618 (1994-04-01), Prober et al.
patent: 5332666 (1994-07-01), Prober et al.
patent: 5720786 (1998-02-01), Hara

Affiliated with

Also associated with

Rating

Computer-aided analysis system for sequencing by hybridization does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Computer-aided analysis system for sequencing by hybridization, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Computer-aided analysis system for sequencing by hybridization will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-762292