RNA amplification method requiring only one manipulation step

Details RNA amplification method requiring only one manipulation step RNA amplification method requiring only one manipulation step

1997-03-31

1998-10-06

Campbell, Eggerton A.

Chemistry: analytical and immunological testing

Process or composition for determination of physical state...

Corrosion resistance or power

435 912, C12Q 168, C12P 1934

Patent

active

058174656

ABSTRACT:
An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.

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