Enzymatic separation of racemic mixtures of hydroxy compounds

Details Enzymatic separation of racemic mixtures of hydroxy compounds Enzymatic separation of racemic mixtures of hydroxy compounds

1983-07-01

1987-04-21

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Resolution of optical isomers or purification of organic...

435195, 435196, C12N 914, C12N 916, C07P 4100

Patent

active

046596712

ABSTRACT:
The invention constitutes an enzymatic method for resolution of a racemic mixture of hydroxy compounds. A D,L mixture of the hydroxy compound is phosphorylated to form the D,L monoorthophosphate esters of the hydroxy compound. Thereafter, the D,L mixture of phosphorylated compounds is treated with a stereospecific phosphatase, such as wheat germ acid phosphatase, which causes the hydrolysis of substantially only one optical isomer of the monoorthophosphate ester. The resulting hydrolyzed isomer of the hydroxy compound is then separated from the remaining monoorthophosphate ester.

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