Synthetic insecticidal gene, plants of the genus oryza transform

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800DIG57, 536 2371, 4353201, 4351723, 43524047, A01H 400, C12N 1532

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054363918

ABSTRACT:
A synthetic gene encoding an insecticidal protein, the gene is provided having a base sequence which has been modified to bring the codon usage in conformity with the genes of gramineous plants particularly rice plants. A preferred gene sequence is shown in SEQ ID NO: 1. An expression vector containing said structural gene is provided. A method for producing recombinant gramineous plants is provided, which comprises transforming protoplasts derived from gramineous plants with the vector, incubating the protoplasts in a liquid medium in the presence of cultured cells of the plants to form colonies and regenerating plants from said colonies. Recombinant gramineous plants having resistance against insects are also provided.

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