Multiple tag labeling method for DNA sequencing

Details Multiple tag labeling method for DNA sequencing Multiple tag labeling method for DNA sequencing

1993-10-26

1995-07-25

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 915, 436 94, 436164, 436172, 436173, 935 77, C12Q 168, C12P 1934, G01N 3350

Patent

active

054361303

ABSTRACT:
A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.

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