Enzyme electrode and assay for determining LDH5

Details Enzyme electrode and assay for determining LDH5 Enzyme electrode and assay for determining LDH5

1987-03-02

1992-09-15

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435817, 435291, 204 111, 204403, G01N 33573, G01N 33577

Patent

active

051477815

ABSTRACT:
The invention relates to an assay for the determination of the enzyme lactate dehydrogenase-5 (LDH5) and to a biosensor for such quantitative determination.
The assay is based on the interaction of this enzyme with the substrate lactic acid and nicotine-amine adenine dinucleotide (NAD) to yield pyruvic acid and the reduction product of NAD.
Anti-LDH5 antibody is bound to a suitable glassy carbon electrode, this is contacted with the substrate containing LDH5, rinsed, inserted into a NAD solution, connected to an amperometric system, lactic acid is added and the current changes are measured, which are indicative of the quantity of LDH-5.

REFERENCES:
patent: 4224125 (1980-09-01), Nakamura et al.
patent: 4224406 (1980-09-01), Gomez et al.
patent: 4321123 (1982-03-01), Nakamura et al.
patent: 4444878 (1984-04-01), Paulus
patent: 4619754 (1986-10-01), Niki et al.
Kenett, J. of Immunoassay, 9(1): 37-49, "Quantitative ELISA For Human Lactate Dehydrogenase Isoenzyme 5" (1988).

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