Asparaginyl tRNA synthetase from streptococcus pneumoniae

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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435 6, 435440, 5303871, 536 232, C12N 900, C12N 1500, C12Q 168, C07H 2104

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06040163&

ABSTRACT:
The invention provides asnS polypeptides and DNA (RNA) encoding asnS polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing asnS polypeptides to screen for antibacterial compounds.

REFERENCES:
R. Calendar et al., "Purification and Physical Characterization of Tyrosyl Ribonucleic Acid Synthetases from Escerichia coli and Bacillus subtilis", Biochemistry, 5(5) p. 1681-1690 (1966).
J. Hughes et al., "How Does Pseudomonas fluorescens, the Producing Organism of the Antibiotic Pseudomonic Acid A, Avoid Suicide?", FEBS Letters, 122(2) p. 322-324 (1980).
Bruand et al., "Nucleotide sequence of the Bacillus subtilis dnaD gene," Microbiology, 141 p. 321-322 (1995).
Seignovert et al., "Asparaginyl-tRNA synthetase from Thermus thermophilus HB8. Sequence of the gene and crystallizationof the enzyme expressed in Escherichia coli", Eur. J. Biochem, 239 p. 501-508 (1996).
Lindler, et al., "Molecular Cloning and Characterization of a Streptococcus DNase Necessary for Repair of DNA Damage Induced by UV Light and Methyl Methanesulfonate", Journal of Bacteriology, 169, (7) pp. 3199-3208 (1987).
Kim et al., "Lactobacillus bulgaricus Asparagine Synthetase and Asparaginyl-tRNA Synthetase: Coregulation by Transcription Antitermination?" Journal of Bacteriology, 178(8) p. 2459-2461 (1996).

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