Process for preparing murine interleukin 2

Details Process for preparing murine interleukin 2 Process for preparing murine interleukin 2

1981-04-14

1983-10-25

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435240, 435241, 435948, 435 41, 435 70, 424177, C12P 2100, C12P 100, C12P 2102, C12N 500, C12N 502, C12R 191, A61K 3700

Patent

active

044119925

ABSTRACT:
A process for preparing murine IL-2 from malignant neoplastic cells includes culturing murine leukemia or lymphoma cells in vitro in a protein containing medium supplemented with various additives. An optimum concentration of a T cell mitogen is added to the culture medium to stimulate maximum production of a supernate which contains IL-2. After a period of time, the supernate is collected and purified into more concentrated form. Phorbol myristate acetate may be added to a suboptimum concentration of the T cell mitogen to reduce the quantity of the mitogen required to produce maximum quantities of murine IL-2.

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Watson et al., "Biochemical and Biological Characterization of Lymphocyte Regulatory Molecules-I. Purification of a Class of Murine Lymphokines", 150 Journal of Experimental Medicine, 849 (1979).
Gillis et al., "Biochemical Characterization of Lymphocyte Regulatory Molecules-II. Purification of a Class of Rat and Human Lymphokines", 124 The Journal of Immunology, 1954 (1980).
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Gillis et al., "Long Term Culture of Tumor Specific Cytotoxic T Cells", 268 Nature, 154 (1977).
Gillis et al., "T-Cell Growth Factor: Parameters of Production and a Quantative Microassay for Activity", 120 The Journal of Immunology, 2027 (1978).

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