Process for the determination of anti-hyaluronidase and an agent

Details Process for the determination of anti-hyaluronidase and an agent Process for the determination of anti-hyaluronidase and an agent

1982-01-26

1983-10-25

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 4, 435 18, 435 36, 435810, 436808, 436811, G01N 3354, C12Q 100, C12Q 134, C12Q 114

Patent

active

044119887

ABSTRACT:
A simple rapid assay for the determination of antihyaluronidase and a kit for use therein. The assay is based on the precipitation of unreacted hyaluronic acid by a cationic detergent and acid dyestuff. The assay is preferably performed as a dilution series. The dyestuff reagent contains 1 to 10 parts by weight acid dyestuff to 10 parts by weight of cationic detergent.

REFERENCES:
patent: 3945889 (1976-03-01), Mima et al.
patent: 4152212 (1979-05-01), Pope et al.
patent: 4373932 (1983-02-01), Gribnau et al.
Ganguly et al., Indian J. Med. Res. 66, 802-808 (1977).
Knoell, Z. Immun.-Forsch. 142, 276-283 (1971).
Ganguly, et al., "Comparative Evaluation of Antihyaluronidase Antistseptolysin O and Streptozyme Test in Acute Rheumatic Activity", Chem. Absts., vol. 88, No. 9 (1978), p. 248, Abs. No. 60739z.
Tolksdorf, "The in vitro Determination of Ayaluronioase,"Meth. Biochem. Anal., vol. 1 (1954), pp. 425-457.
Greiling, "Hyaluronic Acid", Methods of Enzymatic Analysis, vol. 3 (1974), Acadamic Press, N.Y., pp. 1157-1164.
Greiling, et al., "Chondrohin 4-Sulphate, Chondrohin 6-Sulfate and Dermation Sulphate", Methods of Enzymatic Analysis, vol. 3 (1974), Academic Press, N.Y., pp. 1165-1171.

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