Process for the preparation of glutarylacylase in large quantiti

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4353201, 536 232, C12N 978, C12N 1563, C07H 2104

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active

058307435

ABSTRACT:
The gene encoding glutarylacylase (GA) contained in the plasmid pCM 145 (DSM 6409) permits the expression of GA in E. coli in high yields.

REFERENCES:
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Shibuya et al., Agricultural and Biological Chemistry, 45:1561-1567.
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Matsuda et al., "Molecular Cloning and Structure of the Gene for 7beta-(4-Carboxybutanamido)cephalosporanic Acid Acylase from a Pseudomonas Strain," Journal of Bacteriology, vol. 163, No. 3, pp. 1222-1228, Sep., 1985.
Skatrud et al., "Use of Recombinant DNA to Improve Production of Cephalosporin C by Cephalosporium Acremonium," Bio/Technology, vol. 7, pp. 477-485, 1989.
Glover, "Expression of Cloned DNAs in E. coli plasmids" from Gene Cloning: The Mechanics of DNA Manipulation, Chapman and Hall pp. 110-127, 1984.
Mizukami et al., Production of active human interferon-.beta. in E. coli. 1. Preferential poduction by lower culture temperature. Biotechnol. Lett. vol. 8, No. 9, pp. 605-610, 1986, Abstract.
Georgiou, G. "Optimizing the production of recombinant proteins in microorganisms" AIChe Journal (Aug. 1988), vol. 34, No. 8, pp. 1233-1248.
Shibuya et al., "Isolation and Properties of 7.beta.(4-Carboxybutanamido) cephalosporanic Acid Acylase . . . " Agri. Biol. Chem 45(7), pp. 1561-1567, 1981.
Schumacher et al, "Penicillin acylase from E. coli . . . " Nuc. Acids Res. 14(14), pp. 5713-5727, 1986.
Oh et al. "Complete nucleotide sequence of penicillin gacylase . . . " Gene 56, pp. 87-97, 1987.
Matsuda et al. "Molecular Cloning and Structure . . . " J. Bact. 163: p. 1222-1228, 1985.
Glover "Express of cloned DNAs in E. coli plasma" Gene Cloning p. 110-127, 1984.
Skudrud "Use of recombinant DNA to improve . . . " Biotechnology, vol. 7, pp. 477-485, May, 1989.

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