Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-04-26
1998-11-03
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435395, 436178, 536 243, C12Q 168, C12P 1934, H01L 2144, C07H 2104
Patent
active
058306552
ABSTRACT:
The present invention provides modified oligonucleotide primers designed to incorporate a cleavable moiety so that a 3' portion of the primer (linked to an extension product) can be released from an upstream 5' portion of the primer. Upon selective cleavage of the cleavable site, primer extension products that contain about five or fewer base pairs of the primer sequence are released, to provide more useful sizing and sequence information per fragment than extension products containing the entire primer.
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Becker Christopher Hank
Monforte Joseph Albert
Pollart Daniel Joseph
Shaler Thomas Andrew
Evans Susan T.
Fabian Gary R.
Horlick Kenneth R.
SRI - International
Tung Joyce
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