Assay and method for transcript imaging

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2433, C12Q 168, C12P 1934, C07H 2104

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058306480

ABSTRACT:
The invention is directed to assays and methods for transcript imaging. More particularly, the invention is directed to assays and methods for detecting, determining and quantifying the expression level of all known biomolecules in a family of biomolecules, including tyrosine kinases, tyrosine phosphatases and serine/threonine kinases, in uncharacterized biological samples, regardless of species. Such assays and methods may be used as a diagnostic, therapeutic and/or a research tools.

REFERENCES:
patent: 4800159 (1989-01-01), Mullis et al.
Biesecker, Leslie G., et al. "Identification of four murine cDNAs encoding putative protein kinases from primitive embryonic stem cells differentiated in vitro". Proc. Natl. Acad. Sci. USA 90:7044-7048 (1993).
Bishop, Michael J. "The Molecular Genetics of Cancer", Science 235:305-311 (1987).
Cance, William G., et al. "Novel Protein Kinases Expressed in Human Breast Cancer", Int. J. Cancer 54:571-577 (1993).
Elder, James T., et al. "Overexpression of Transforming Growth Factor .alpha. in Psoriatic Epidermis". Science 243:811-814 (1989).
Feng, Gen-Sheng, et al. "SH2-Containing Phosphotyrosine Phosphates as a Target of Protein-Tyrosine Kinases", Science 259: 1607-1611 (1993).
Fry, David W. "Protein tyrosine kinases as therapeutic targets in cancer chemotherapy and recent advances in the development of new inhibitors". Exp. Opin. Invest. Drugs 3(6):577-595 (1994).
Hanks, Steven K., et al. "The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Cataytic Domains", Science 241:42-52 (1988).
Hynes, N.E. and Stern, D.F. "The Biology of erbB-2
eu/HER-2 and it Role in Cancer", Biochim Biophys Acta 1198:165-184 (1994).
Krueger, Neil X., et al. "Structural diversity and evolution of human receptor-like protein tyrosine phosphates". The EMBO Journal 9(10):3241-3252 (1990).
Lai, Cary and Lemke, Greg "An Extended Family of Protein-Tyrosine Kinase Genes Differentially Expressed in the Vertebrate Nervous System". Neuron 6:691-704 (1991).
Levitzki, Alexander and Gazit, Aviv "Tyrosine Kinase Inhibition: An Approach to Drug Development". Science 267:1782-1788 (1995).
Maher, Pamela A. and Pasquale, Elena B. "Tyrosine Phosphorylated Proteins in Different Tissues during Chick embryo Development", The Journal of Cell Biology 106:1747-1755 (1988).
Marcella, C. and Eichmann A. "Molecular cloning of a family of protein kinase genes expressed in the avian embryo". Oncogene 7:2479-2487 (1992).
Ninfa, Elizabeth G. and Dixon, Jack E. "Protein tyrosine phosphates in disease processes". Trends in Cell Biology 4:427-430 (1994).
Pasquale, Elena B. "A distinctive family of embryonic protein-tyrosine kinase receptors". Proc. Natl. Acad. Sci USA 87:5812-5816 (1990).
Partanen, Juha, et al. "Putative tyrosine kinases expressed in K-562 human leukemia cells". Proc. Natl. Acad. Sci. USA 87:8913-8917 (1990).
Ross, Russell "Platelet-Derived Growth Factor". The Lancet 1179-1182 (1989).
Sahin, Mustafa and Hockfield, Susan "Protein Tyrosine Photophatases Expressed in the Development Rat Brain". The Journal of Neuroscience 13(11):4968-4978 (1993).
Saiki, Randall K., et al. "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase". Science 239:487-491 (1988).
Saiki, Randall K., et al. "Enzymatic Amplification of .beta.-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia". Science 230:1350-1354 (1985).
Sanchez, Marina P., et al. "Multiple tyrosine protein kinases in rat hippocampal neurons: Isolation of Ptk-3, a receptor expressed in proliferative zones of the developing brain". Proc. Natl. Acad. Sci. USA 1891:819-1823 (1994).
Schepens, Jan, et al. "Identification and typing of members of the protein-tyrosine phosphatase gene family expressed in mouse brain". Molecular Biology Reports 16:241-248 (1992).
Schlesinger, J. and Ullrich, A. "Growth Factor Signaling by Receptor Tyrosine Kinases". Neuron 9:383-391 (1992).
Shen, Shi-Hsiang, et al. "A protein-tyrosine phosphatase with sequence similarity to the SH2 domain of the protein-tyrosine kinases". Nature 352:736-739 (1991).
Shock, Lisa P., et al. "Protein tyrosine phosphatases expressed in developing brain and retinal Muller glia". Molecular Brain Research 28:110-116 (1995).
Siyanova, E.Y., et al. "Tyrosine kinase gene expression in the mouse small intestine". Oncogene 9:2053-2057 (1994).
Tonks, Nicholas K., et al. "Purification of the Major Protein-tyrosine phosphatases of Human Placenta". The Journal of Biological Chemistry 263(14):6722-6730 (1988).
Vassar et al. "Transgenic mice provide new insights into the role of TGF-.alpha. during epidermal development and differentiation", Genes and Development 5:714-727 (1991).
Vogel et al. "Activation of a Phosphototyrosine Phosphatase by Tyrosine Phosphorylation". Science 259:1611-1614 (1993).
Wilks et al. "The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family". Gene. 85:67-74 (1989).
Wilks. "Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction". Proc. Natl. Acad. Sci. USA 86:1603-1607 (1989).
Yang et al. "Isolation of a cDNA clone encoding a human protein-tyrosine phosphatase with homology to the cytoskeletal-associated proteins band 4.1, ezrin, and talin". Proc. Natl. Acad. Sci USA 88:5949-5953 (1991).
Guersen et al. Population study of T cell receptor V-beta gene usage in peripheral blood lymphocytes: differences in ethnic groups Clin. Exp. Immunol. vol. 94 201-207, 1993.
Werner et al. Experimental diabetes increases insulinlike growth factor I and II receptor concentration and gene expression in kidney Diabetes vol. 39 1490-1497, 1990.
Ikeda et al. Expression and functional role of the proto-oncogene c-kit in acute myeloblastic leukemic cells Blood vol. 78 2962-2968, 1991.
Boehm Analysis of multigene families by DNA fingerprinting of conserved domains: directed cloning of tissue-specific protein tyrosine phosphatases Oncogene vol. 8 1385-1390, 1993.
Caldwell et al. Surface and cytoplasmic expression of CD45 antigen isoforms in normal and malignant myeloid cell differentiation Am. J. Hematol. vol. 95 180-187, 1991.
Cohen et al. Interleukin 6 induces myeloid differentiation of a human biphenotypic leukemic cell line Leukemia Research vol. 16 751-760, 1992.
Hendriks et al. Rapid assessment of protein-tyrosine phosphatase expression levels by RT-PCR with degenerate primers. Mol. Biol. Reports vol. 19 105-108, 1994.
Marguerie et al. PCR-based analysis of the TCR repertiore in human autoimmune diseases. Immunology Today vol. 13 pp. 336-338, 1992.

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