Tryptamine producing tryptophan decarboxylase gene of plant orig

Details Tryptamine producing tryptophan decarboxylase gene of plant orig Tryptamine producing tryptophan decarboxylase gene of plant orig

1995-04-20

1998-09-29

Vogel, Nancy T.

Chemistry: molecular biology and microbiology

Plant cell or cell line, per se ; composition thereof;...

Plant cell or cell line, per se, contains exogenous or...

4352523, 43525233, 536 232, 536 2431, C12N 504, C12N 121, C12N 1560

Patent

active

058145206

ABSTRACT:
In accordance with the present invention, there is provided an isolated and purified DNA fragment comprising a DNA sequence encoding a plant decarboxylase. Preferably, the DNA sequence encodes a plant tryptophan decarboxylase, more preferably from Catharanthus roseus. A 1747 bp cDNA clone was isolated by antibody screening of a cDNA expression library produced from poly A.sup.+ RNA found in developing seedlings of C. roseus. The invention also includes DNA sequences encoding a plant tryptophan decarboxylase which are synthetically produced to correspond substantially to the isolated and purified DNA sequence encoding the same enzyme. The nucleotide sequence of the synthetic DNA sequence is determined on the basis of codon degeneracy.

REFERENCES:
Noe et al 1984 Plant Mol. Biol. vol. 3 281-288.
Maniatis et al 1982 Molecular Cloninic, Sect pp. 212-246.
De Luca et al. 1989 PNAS 86:2582-2586.
Broom et al. PNAS 1978 (75):2746-2749.
Albert, V.R., et al., "A Single Gene Codes for Aromatic L-Amino Acid Decarboxylase in Both Neuronal and Non-Neuronal Tissues," J. Biol. Chem., 262:9404-9411 (1987).
Broom, et al., Proc. Natl. Acad. Sci. USA, 75: 2746-2749 (1978).
Chavadez, S., et al., "Production of Transgenic Plants which Accumulate Tryptamine Through Expression of Tryptophan Decarboxylase," International Society for Plant Molecular Biology, Third International Congress, Abstract 1098 (1991).
De Luca, et al., "Molecular Cloning and Analysis of cDNA Encoding a Plant Tryptophan Decarboxylase: Comparison with Animal dopa Decarboxylases," Proc. Natl. Acad. Sci. USA, 86: 2552-2556 (1989).
Eveleth, D.D., et al., "Sequence and Structure of the dopa Decarboxylase Gene of Drosophila: Evidence for Novel RNA Splicing Variants," EMBO J., 5:2663-2672 (1986).
Facchini, P.J., and V. De Luca, "Differential and Tissue-Specific Expression of a Gene Family for Tyrosine/Dopa Decarboxylase in Opium Poppy," J. Biol. Chem., 269:26684-26690 (1994).
Fecker, L., et al., "Expression of a Bacterial Lysine Decarboxylase Gene in Tobacco," International Society for Plant Molecular Biology, Third International Congress, Abstract 1092 (1991).
Joh, T.H., et al., "Molecular Biology of Aromatic L-Amino Acid Decarboxylase and B-Hydroxylase," Progress in Catecholamine Research, Part a: Basic Aspects And Peripheral Mechanisms, pp. 29-34 (1988).
Maniatis, et al., Molecular Cloning, pp. 212-246 (1982).
Noe, W., et al., "Tryptophan Decarboxylase from Catharanthus roseus Cell Suspension Cultures: Purification, Molecular and Kinetic Data of the Homogenous Protein," Plant Mol. Biol. vol. 3, pp. 281-288 (1984).
Songstad, D.D., et al., "High Levels of Trytamine Accumulation Accumulation in Transgenic Tobacco Expressing Tryptophan Decarboxylase," Plant Physiol., 94:1410-1413 (1990).

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