Oligonucleotide N3'-P5' phosphoramidates: hybridization and nucl

Details Oligonucleotide N3'-P5' phosphoramidates: hybridization and nucl Oligonucleotide N3'-P5' phosphoramidates: hybridization and nucl

1994-03-18

1997-02-04

Elliott, George C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, 435 6, C07H 2104, C12Q 168

Patent

active

055999220

ABSTRACT:
Modified oligonucleotide 3'-NHP(O)(O.sup.-)O-5' phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these compounds form more stable duplexes than phosphodiesters with complementary DNA and particularly RNA strands. Further, the phosphoramidate analogs can also form stable triplexes with double-stranded DNA target, where under similar conditions parent phosphodiester compounds failed to do so.

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