Fluorometric system, method and test article

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250302, 250373, 424 12, A61K 3900, G01T 120

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039926311

ABSTRACT:
A fluorometric system to determine the kind and amount of substances derived from a biological fluid (e.g., serum or urine) or tissue. The substances to be detected (e.g., antigen, antibody, hormone or enzyme) is coated onto a substrate in fluorescent form. Multiple coating areas of different samples may be employed. The fluorometric system includes a source of light to excite fluorescence, a fiber optic cable to conduct the excitation light to such coating, and a second fiber optic cable to conduct emitted fluorescence to a detector device. The system minimizes any gap distance in the path from the sample to the detector which permits the loss of excessive fluorescence. A branched fiber optical cable with the main trunk terminating adjacent the sample with one branch for transmitting light to the sample and the other for transmitting fluorescence light to the detector.

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