Antibiotics Y-16482 .alpha. and/or Y-16482 .beta., a process for

Details Antibiotics Y-16482 .alpha. and/or Y-16482 .beta., a process for Antibiotics Y-16482 .alpha. and/or Y-16482 .beta., a process for

1982-02-19

1984-04-03

Goldberg, Jerome D.

Drug, bio-affecting and body treating compositions

Fermentate of unknown chemical structure

Having a known elemental analysis

424119, 435170, A61K 3500, C12P 104

Patent

active

044407521

DESCRIPTION:

BRIEF SUMMARY
TECHNICAL FIELD

The present invention relates to novel antibiotics Y-16482 .alpha. and/or Y-16482 .beta., a process for production thereof, and a pharmaceutical composition containing either or both of them.


DISCLOSURE OF INVENTION

In an attempt of obtaining novel antibiotics, the present inventors, while obtaining many soil microorganisms and isolating antibiotic substances produced by them, came to note that a particular strain belonging to the genus Pseudomonas, when cultivated in a proper medium, accumulated a substance having a strong inhibitory activity against gram-positive as well as gram-negative bacteria and also showing antitumor activity. The isolation and identification of the physicochemical and biological characters of the substance led to the finding that the product was a novel antibiotic and it was named as Antibiotic Y-16482.
Further the present inventors found that Antibiotic Y-16482 consisted of two components, obtained them separately by a purification method as mentioned hereinafter, and named them as Antibiotics Y-16482 .alpha. and Y-16482 .beta., respectively (hereafter may be referred to briefly as Y-16482 .alpha. and Y-16482 .beta., respectively).
The present invention relates to (1) Antibiotics Y-16482 .alpha. and/or Y-16482 .beta. and their salts, (2) Process of producing Antibiotics Y-16482 .alpha. and/or Y-16482 .beta., characterized by cultivating a strain belonging to the genus Pseudomonas and capable of producing Antibiotic Y-16482, thereby to cause it to accumulate Antibiotics Y-16482 .alpha. and/or Y-16482 .beta. in a medium and recovering them, and (3) a pharmaceutical composition characterized in containing Antibiotics Y-16482 .alpha. and/or Y-16482 .beta. as the effective ingredients.
All references to tumors throughout this disclosure are applicable to transplanted tumors.


BRIEF EXPLANATION OF DRAWINGS

FIG. 1 indicates the ultraviolet absorption spectrum of hydrochloride of Antibiotic Y-16482 .alpha. as measured in a methanolic solution (concentration: 25.4 mcg/ml);
FIG. 2 indicates the infrared absorption spectrum of hydrochloride of Antibiotic Y-16482 .alpha. as measured by KBr method;
FIG. 3 indicates the ultraviolet absorption spectrum of hydrochloride of Antibiotic Y-16482 .beta. as measured in a methanolic solution (concentration: 20.6 mcg/ml) and
FIG. 4 indicates the infrared absorption spectrum of hydrochloride of Antibiotic Y-16482 .beta. as measured by KBr method.


DETAILED EXPLANATION OF INVENTION

As the antibiotic Y-16482-producing microorganism, any strain may be utilized, which belongs to the genus Pseudomonas and is capable of producing Antibiotics Y-16482 .alpha. and/or Y-16482 .beta.. For example, a strain A2-2 of Pseudomonas fluorescens (hereinafter may be briefly referred to as A2-2 strain) is applicable, which the present inventors isolated from a soil sample obtained at the foot of Mt. Hikosan, in Soeda-cho, Tagawa-gun, Fukuoka.
The microbiological characters of A2-2 strain are as follows:


1. Morphology

The cell grown in Bouillon broth medium and on Bouillon agar medium showed the following morphology: cells irregular, no chain formed, mostly occuring singly but some in pairs. method. incubation at 30.degree. C.


2. Observation of growth on various media
hrs' incubation it grew into 1 to 1.5 mm in diameter, circular in entire, smooth on surface, pale yellow, translucent, wet, a little glistening. No water-solble pigment produced. yellow, translucent, wet, dull glistening. 30.degree. C. and sedimentation occurred at the bottom of the test tube. No pellicle produced. incubation at 20.degree. to 21.degree. C., and liquefied in infundibule until the third day and then the liquefaction gradually proceeded in stratiform.


3. Physiological characters



______________________________________ Psedomonas, A2-2 fluorescens. strain biotype A ______________________________________ Test items 1. Reduction of nitrate - - 2. Denitrification - - 3. MR test - - 4. VP test - - 5. Production of indole - - 6. Productio

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