Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor
Patent
1985-06-06
1989-04-11
Tarcza, John E.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including perfusion; composition therefor
424 93, 623 1, 435240, 435242, 43524021, A01N 0102, A61K 4900, A61K 3544, C12N 0500
Patent
active
048206261
ABSTRACT:
The present invention provides a novel method of treating a synthetic or naturally occurring implant, such as a vascular graft, intended for implantation in a human patient, comprising obtaining human microvascular rich tissue from that patient; separating microvascular endothelial cells from that tissue; and placing said microvascular endothelial cells onto said implant to provide at least about 50% confluence of said cells on the surface of said implant to be treated. In the preferred embodiment, the microvascular rich tissue is perinephric fat, which is obtained from the donor and subjected to a digestive separation process to provide an abundant supply of microvascular endothelial cells. In the preferred embodiment, these microvascular endothelial cells are placed onto the implant by suspending them in a protein (plasma) containing physiologic saline solution, which is incubated on the graft until the endothelial cells either adhere to the graft surface or clot within the protein solution. An efficient method is therefore provided which will permit a patient to donate excess microvascularized tissue which may be used to procure autologous endothelial cells which are subsequently used to treat a graft or other implant to provide at least 50% confluence while his related surgical procedure is in progress.
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Jarrell Bruce E.
Williams Stuart K.
Tarcza John E.
Thomas Jefferson University
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