Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-02-26
2000-08-01
Elliot, George C.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 4353201, 435 911, 435 913, 536 231, 536 243, 536 245, 536 2433, C12P 1934, C12Q 168, C07H 2102, C07H 2104
Patent
active
06096880&
ABSTRACT:
The present invention provides methods for synthesis, and therapeutic use of DNA and RNA oligonucleotides and analogs. RNA oligonucleotides arc synthesized using a small, circular DNA template which lacks an RNA polymerase promoter sequence. The RNA synthesis is performed by combining a circular single-stranded oligonucleotide template with an effective RNA polymerase and at least two types of ribonucleotide triphosphate to form an RNA oligonucleotide multimer comprising multiple copies of the desired RNA oligonucleotide sequence. Preferably, the RNA oligonucleotide multimer is cleaved to produce RNA oligonucleotides having well-defined ends. Preferred RNA oligonucleotide multimers contain ribozymes capable of both cis (autolytic) and trans cleavage.
REFERENCES:
patent: 4795700 (1989-01-01), Dervan et al.
patent: 4837312 (1989-06-01), Dervan et al.
patent: 4987071 (1991-01-01), Cech et al.
patent: 5093246 (1992-03-01), Cech et al.
patent: 5246921 (1993-09-01), Reddy et al.
patent: 5258506 (1993-11-01), Urdea et al.
patent: 5354668 (1994-10-01), Auerbach
patent: 5354855 (1994-10-01), Cech et al.
patent: 5426180 (1995-06-01), Kool
patent: 5470724 (1995-11-01), Ahern
patent: 5498531 (1996-03-01), Jarrell
patent: 5500357 (1996-03-01), Taira et al.
patent: 5648245 (1997-07-01), Fire et al.
patent: 5714320 (1998-02-01), Kool
Branch A., "A Good Antisense is Hard to Find" TIBS vol. 23:45-50, Feb. 1998.
Stull et al., "Antigene, Ribozyme, and Aptamer Nucleic Acids Drugs: Progress and Prospects" Pharmaceutical Research vol. 12(4) 465-483, 1995.
Agrawal S., "Antisense Oligonucleotides: Towards Clinical Trials" TIBTECH vol. 14:376-387, Oct. 1996.
C. M. Flory et al., "Nuclease-Resistant Ribozymes Decrease Stromelysin mRNA Levels in Rabbit Synovium Following Exogenous Delivery to the Knee Joint," Proc. Natl. Acad. Sci. USA, 93 754-758 (1996).
M. Kashani-Sabat et al., "Reversal of the Malignant Phenotype by an Anti-ras Ribozyme," Antisense Research and Development, 2:3-15 (1992).
S. B. Noonberg et al., "Characteristics of Oligonucleotide Uptake in Human Keratinocyte Cultures," Journal of Investigative Dermatology, 101 727-731 (1993).
M. Sioud, "Ribozyme Modulation of Lipopolysaccharide-Induced Tumor Necrosis Factor-.varies. Production by Peritoneal Cells in vitro and in vivo," Eur. J. Immunol., 26 1026-1031 (1996).
B. M. Chowrira et al., "In Vitro and In Vivo Comparison of Hammerhead, Hairpin, and Hepatitis Delta Virus Self-Processing Ribozyme Cassettes," Journal of Biological Chemistry, 269 25856-25864 (1994).
A. M. Diegelman et al., "Generation of Circular RNAs and Trans-Cleaving Catalytic RNAs by Rolling Transcription of Circular DNA Oligonucleotides Encoding Hairpin Ribozymes," Nucleic Acids Research, 26 3235-3241 (1998).
G. Krupp, "Unusual Promoter-Independent Transcription Reactions with Bacteriophage RNA Polymerases," Nucleic Acids Research, 17 3023-3036 (1989).
L. M. Hoffman et al., "Enzymatic Synthesis of Milligram Quantities of Ribozymes in Small Volumes," BioTechniques, 17 372-375 (1994).
M. Koizumi et al., "Ribozymes Designed to Inhibit Transformation of NIH3T3 Cells by the Activated c-Ha-ras Gene," Gene, 117 179-184 (1992).
D. Liu et al., "Rolling Circle DNA Synthesis: Small Circular Oligonucleotides as Efficient Templates for DNA Polymers," J. Am. Chem. Soc., 118: 1587-1594 (1996).
S. L. Daubendiek et al., "Generation of Catalytic RNAs by Rolling Transcription of Synthetic DNA Nanocircles," Nature Biotech., 15: 273-277 (Mar. 1997).
K. Tiara et al., "Construction of a Novel Artificial-Ribozyme-Releasing Plasmid," Protein Engineering, 3: 733-737 (1990).
S. L. Daubendiek et al., "Rolling-Circle RNA Synthesis: Circular Oligonucleotides as Efficient Substrates for T7 RNA Polymerase," No. 112625x of Chem. Abstr., 123: 1175 (1995).
E.T. Kool, "Circular Oligonucleotides: New Concepts in Oligonucleotide Design," Annu. Rev. Biophys. Biomol. Struct., 25: 1-28 (1996).
E. T. Kool, "Topologically Modified Biopolymers--Properties of Synthetic Circular DNAs and RNAs," Trends in Polymer Science, 3: 396-402 (1995).
"Affinity Chromatography: Practical and Theoretical Aspects", Ed. P. Mohr: Dekker Publishing; New York (1985); Title page, Copyright page, and Contents pages (pp. v-viii).
Aguilar, L. et al., "Hairpin, Dumbbell, and Single-Stranded Phosphodiester Oligonucleotides Exhibit Identical Uptake in T. Lymphocyte Cell Lines", Antisense & Nucleic Acid Drug Development, 6, 157-163 (1996).
Aiyar, S. E. et al., "A Mismatch Bubble in Double-stranded DNA Suffices to Direct Precise Transcription Initiation by Escherichia coli RNA Polymerase", J. Biol. Chem., 269(18), 13179-13184 (1994).
Albrecht, T. et al., "Cationic lipide mediated transfer of c-abl and bcr antisense oligonucleotides to immature normal myeloid cells: Uptake, biological effects and modulation of gene expression", Ann. Hematol., 72, 73-79 (1996).
Ashley, G.W., et al., "Chemical Synthesis of Oligodeoxynucleotide Dumbbells", Biochemistry, 30, 2927-2933 (1991).
Beaucage, S.L., et al., "Deoxynucleoside Phosphoramidites--A New Class of Key Intermediates for Deoxypolynucleotide Synthesis", Tetrahedron Lett., 22(20), 1859-1862 (1981).
Blanco, L. et al., "Highly Efficient DNA Synthesis by the Phage .phi.29 DNA Polymerase", J. Biol. Chem., 264(15), 8935-8940 (1989).
Bock, L.C., et al., "Selection of single-stranded DNA molecules that bind and inhibit human thrombin", Nature, 355, 564-566 (1992).
Capaccioli, S. et al., "Cationic Lipids Improve Antisense Oligonucleotide Uptake and Prevent Degradation in Cultured Cells and in Human Serum", Biochemical and Biophysical Research Communications, 197(2), 818-825 (1993).
Chin, J., et al., "Catalytic Hydrolysis of Amides at Neutral pH", J. Chem. Soc., Chem. Commun., 1326-1328 (1990).
Compton, J., "Nucleic acid sequence based amplification", Nature, 350, 91-92 (1991).
Cwirla, S. E. et al., "Peptides on phage: A vast library of peptides for identifying ligands", PNAS USA, 87, 6378-6382 (1990).
Daube, S. S. et al., "Functional Transcription Elongation Complexes from Synthetic RNA-DNA Bubble Duplexes", Science, 258, 1320-1324 (1992).
Daubendiek, S. L., et al., "Rolling-Circle RNA Synthesis: Circular Oligonucleotides as Efficient Substrates for T7 RNA Polymerase", J. Am. Chem. Soc., 117, 7818-7819 (1995).
D'Souza, D. J. et al., "Strong Binding of Single-stranded DNA by Stem-Loop Oligonucleotides", J. Biomolecular Structure and Dynamics, 10(1), 141-152 (1992).
Dzianott, A. M., et al., "Derivation of an infectious viral RNA by autolytic cleavage of in vitro transcribed viral cDNAs", PNAS USA, 86, 4823-4827 (1989).
Eisenberg, S. et al., "The Single-Stranded DNA Phages", Eds., Denhardt, D.T., Cold Spring Harbor Press, Cold Spring Harbor, Title page, Copyright Page, Contents Pages, pp. 298-299 (1978).
Eisenberg, S., et al., "Enzymatic replication of viral and complementary strands of duplex DNA of phage .phi.X174 proceeds by separate mechanisms", PNAS USA, 73(9), 3151-3155 (1976).
Ellington, A. D., et al., "In vitro selection of RNA molecules that bind specific ligands", Nature, 346, 818-822 (1990).
Ellington, A. D., et al., "Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures", Nature, 355, 850-852 (1992).
Famulok, M., et al., "Stereospecific Recognition of Tryptophan Agarose by in vitro Selected RNA", J. Am. Chem. Soc., 114, 3990-3991 (1992).
Fire, A., et al., "Rolling replication of short DNA circles", PNAS USA, 92, 4641-4645 (1995).
Forster, A.C. et al., "Structural and Ionic Requirements for Self-cleavage of Virusoid RNAs and trans Self-cleavage of Viroid RNA", Cold Spring Harbor Symposia on Quantitative Biology, LII, 249-259 (1987).
Grosshans, C. A., et al., "A hammerhead ribozyme allows synthesis of a new form of the Tetrahymena ribozyme homogeneous in length with a 3' end blocked for transesterification", Nucleic Acids Research, 19(14), 3875-3880 (1991).
Gura, T., "Antisense Has Growing Pains", Science, 270, 575-577 (1995).
Guy-Caffey, J. K., et al., "Novel Polyaminolipids Enhance the Cellular Uptake of Oligonucleotides", J. Biol. Chem., 270(52), 31391-313
Elliot George C.
McGarry Sean
University of Rochester
LandOfFree
Circular DNA vectors for synthesis of RNA and DNA does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Circular DNA vectors for synthesis of RNA and DNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Circular DNA vectors for synthesis of RNA and DNA will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-665244