Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai
Patent
1996-11-08
2000-08-01
Priebe, Scott D.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Carbohydrate doai
435 697, 435325, 435419, 435455, 435468, 435 911, 800 8, C12N 1579, C12N 510, C12P 1934, C12P 2100
Patent
active
06096717&
ABSTRACT:
The invention described here is a method whereby a molecular tag is put on a gene, transcript and protein in a single recombinational event. The protein tag takes the form of a unique peptide that can be recognized by an antibody or other specific reagent, the transcript tag takes the form of the sequence of nucleotides encoding the peptide that can be recognized by a specific polynucleotide probe, and the gene tag takes the form of a larger sequence of nucleotides that includes the peptide-encoding sequence and other associated nucleotide sequences. The central feature of the invention in its essential form is that the tag-creating DNA has a structure such that when it is inserted into an intron within a gene it creates two hybrid introns separated by a new exon encoding the protein tag. A major virtue of the method is that it allows one to identify new proteins or protein-containing structures, and, having done so, to readily identify and analyze the genes encoding those proteins.
REFERENCES:
Theiler, K., The House Mouse: Atlas of Embryonic Development, Springer-Verlag: New York, pp. 148-149, 1989.
Debuchy et al., "The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus," The EMBO J., 8: 2803-2809 (1989).
Diener et al., "Rescue of a paralyzed flagella mutant of Chlamydomonas by transformation," Proc. Nat. Acad. Sci. USA, 87: 5739-5743 (1989).
Karem et al., Proc. Nat. Acad. Sci., USA 87, 8447-8451 (1990).
Kindle, High frequency nuclear transformation of Chlamydomonas reinhardtii, Proc. Nat. Acad. Sci. USA 87: 1228-1232 (1990).
Kornfeld et al., Structure and expression of a family of Ultrabithorax mRNAs generated by alternative splicing and polyadenylation in Drosophila, Genes Dev 3: 243-258 (1989).
Kransnow et al., "Transcriptional activation and repression by Ultrabithorax proteins in cultured Drosophila Cells," Cell 57: 1031-1043 (1989).
Nilsson et al., Journal of Cell Biology 120: 5-13 (Jan. 1993).
O'Conner et al., "Alternative products from the Ultrabithorax domain of the bithorax complex," EMBO J., 7: 435-455 (1988).
Sanger et al., "DNA sequencing with chain-terminating inhibitors," Proc. Nat. Acad. Sci. USA, 74: 5463-5467 (1977).
Silflow et al., "Chlamydomonas reinhardtii tubulin gene structure," Ann. N.Y. Acad. Sci., 466: 18-30 (1986).
Soldati et al., Cell 66: 277-289 (1991).
Taillon et al., "Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas," J. Cell Biol., 119: 1613-1623 (1992).
Weber et al., Cell 36: 983-992 (1984).
Williams et al., "Molecular cloning and sequence analysis of the Chalamydomonas gene coding for radial spoke protein 3: flagellar mutation pf-14 is an ochre allele," J. Cell Biol., 109: 235-245 (1989).
Wilson et al., "The structure of an antigenic determinant in a protein," Cell 37: 767-778 (1984).
Wright et al., "A nucleus/basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segration," J. Cell Biol., 101: 1903-1912 (1985).
Adams et al., "Complimentary DNA sequencing: expressed sequence tags and Human Genome Project," Science, 252: 1651-1656 (1991).
Adams et al., "Sequence identification of 2.375 human brain genes," Nature 355: 632-634 (1992).
Botstein et al., "Construction of a genetic linkage map in man using restriction fragment length polymorphisms," Am. J. Hum. Genet. 32: 314-331 (1980).
Charbit et al., Probing the topology of a bacterial membrane protein by genetic insertion of a foreign epitope: expression at the cell surface. EMBO J., 5: 3029-3037 (1986).
Chen et al., "High-efficiency transformation of mammalian cells by plasmid DNA," Mol. Cell. Biol. 7: 2745-2752 (1987).
Colbere-Garapin et al., "Patterns of integration of exogenous DNA sequences transfected into mammalian cells of primate and rodent origin," Gene, 50: 279-288 (1986).
Cooley et al., "Insertional mutagenesis of the Drosophila genome with single P elements," Science, 239: 1121-1128 (1988).
Craik et al., "Splice junctions: association with variations in protein structure," Science, 220: 1125-1129 (1983).
Duyk et al., Exon trapping: a genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA, Proc. Nat. Acad. Sci. USA, 87: 8995-8999 (1990).
Field et al., "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method," Mol. Cell Biol., 8: 2159-2165.
Freimuth et al., "Codon insertion mutants of the adenovirus terminal protein," Proc. Nat. Acad. Sci. 7816-7820 (1986).
Freimuth et al., "Introduction of guest peptides into Escherichia coli alkaline phostase," J. Biol. Chem., 265: 896-901 (1990).
Friedrich et al., "Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice," Genes and Dev., 5: 1513-1523 (1991).
Gossler et al., "Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes," Science, 244: 463-465 (1989).
Green et al., "Biochemical mechanisms of constitutive and regulated pre-mRNA splicing," Ann. Rev. Cell Biol., 7: 559-599 (1991).
Grimwade et al., "Gene expression and protein localization in yeast," 16th Int. Conf. on yeast genetics and molecular biology: S695, (1992).
Hawkins, "A survey on intron and exon lengths," Nucleic Acids Res., 16: 9893-9910 (1988).
Hochgeschwender et al., "Identifying genes within the genome: new ways for finding the needle in the haystack," BioEssays, 13: 139-144 (1991).
Lobel, "Construction of mutants of Malony murine leukemia virus by suppressor-linked Insertonal mutagenesis: positions of viable insertion mutations," Genetics 81: 4149-4153 (1984).
Luehrsen et al., "Insertion of non-intron sequence into maize introns interferes with Splicing," Nucl. Acids Res., 20: 5181-5187.
Munro et al., "Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophilia hsp70," EMBO J., 3: 3087-3093 (1984).
Murnane et al., Recombination events during integration of transfected DNA into normal human cells, Nucleic Acids Res., 18: 2733-2738 (1990).
O'Kane et al., Detection in situ of genomic regulatory elements in Drosophilia, Proc. Nat. Acad. Sci. USA, 84: 9123-9127 (1987).
Rommens et al., "Identification of the cystic fibrosis gene: chromosome walking and jumping," Science 245: 1059-1065 (1989).
Snyder et al., ".lambda.gt11: gene isolation with antibody probes and other applications," Methods in Enzymology, 154: 107-128 (1987).
LandOfFree
Method for producing tagged genes transcripts and proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for producing tagged genes transcripts and proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing tagged genes transcripts and proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-663951