Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-03-30
2000-08-01
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 71, 436501, 436504, 536 221, 536 231, 935 77, 935 82, C12Q 168, C12Q 170, C07H 1900, C07H 2102
Patent
active
060965029
ABSTRACT:
A UL9 substrate for detecting helicase activity in a UL9 protein is provided. In one embodiment, the substrate includes a first strand including a herpes replication origin sequence and a first single stranded tail; and a second strand including a sequence complementary to the herpes replication origin sequence. In one variation, the first single stranded tail is 3' relative to the herpes replication origin sequence. The second strand may optionally further include a single stranded tail. The single stranded tail on the second strand may be 3' or 5' relative to the sequence complementary to the herpes replication origin sequence. In another variation, the first single stranded tail is 5' relative to the herpes replication origin sequence. The second strand may optionally further include a single stranded tail. The single stranded tail on the second strand may be 3' or 5' relative to the sequence complementary to the herpes replication origin sequence. The UL9 substrate may optionally further include at least one detectable marker attached to at least one of the first and second strands. The UL9 substrate may also optionally be attached to a solid support.
REFERENCES:
Fierer, D. et al., "The Stoichiometry of Binding of the Herpes Simplex Virus Type Origin Binding Protein, UL9, to Ori.sub.s ", The Journal of Biological Chemistry, vol. 270, No. 13, Mar. 31, 1995, pp. 7330-7334.
Boehmer, P. et al., "Association of Origin Binding Protein and Single Strand DNA-binding Protein, ICP8, during Herpes Simplex Virus Type 1 DNA Replication in Vivo", The Journal of Biological Chemistry, vol. 269, No. 46, Nov. 18, 1994, pp. 29329-29334.
Lee, Sam S.-K. et al., "Unwinding of the box I element of a herpes simplex virus type 1 origin by a complex of the viral origin binding protein, single-strand DNA binding protein, and single-stranded DNA", Proc. Natl. Acad. Sci. USA, vol. 94, pp. 2838-2842, Apr. 1997.
Weir, H. et al., "Binding of the herpes simplex virus type 1 UL9 gene product to an origin of viral DNA replication", Nucleic Acids Research, vol. 17, No. 4, (1989), pp. 1429-1425.
Gustafsson, C. et al., "The DNA Ligands Influence the Interactions between the Herpes Simplex Virus 1 Origin Binding Protein and the Single Strand DNA-binding Protein, ICP-8", The Journal of Biological Chemistry, vol. 270, No. 32, Aug. 11, 1995, pp. 19028-19034.
Elias, P. et al., "Structural Elements Required for the Cooperative Binding of the Herpes Simplex Virus Origin Binding Protein to oriS Reside in the N-Terminal Part of the Protein", The Journal of Biological Chemistry, vol. 267, No. 24, Aug. 25, 1992, pp. 17424-17429.
Stabell, E. et al., "A truncated herpes simplex virus origin binding protein which contains the carboxyl terminal origin binding domain binds to the origin of replication but does not alter its conformation", Nucleic Acids Research, vol. 21, No. 22, (1993), pp. 5203-5211.
Elias, P. et al., "The Origin Binding Protein of Herpes Simplex Virus 1 Binds Cooperatively to the Viral Origin of Replication Ori.sub.s ", The Journal of Biological Chemistry, vol. 265, No. 28, Oct. 5, 1990, pp. 17167-17173.
Olivo, P. et al., "Herpes simplex virus DNA replication: The UL9 gene encodes an origin-binding protein", Proc. Natl. Acad. Sci. USA, vol. 85, pp. 5414-5418, Aug. 1988.
Weir, H. et al., "Two binding sites for the herpes simplex virus type 1 UL9 protein are required for efficient activity of the ori.sub.s replication origin", Journal of General Virology (1990), vol. 71, pp. 1379-1385.
Hazuda, D. et al., "Cooperative Interactions between Replication Origin-bound Molecules of Herpes Simplex Virus Origin-binding Protein Are Mediated via the Amino Terminus of the Protein", The Journal of Biological Chemistry, vol. 267, No. 20, Jul. 15, 1992, pp. 14309-14315.
Elias, P. et al., "A DNA binding protein specific for an origin of replication of herpes simples virus type 1", Proc. Natl. Acad. Sci. USA, vol. 83, pp. 6322-6326, Sep. 1986.
Earnshaw, D. et al., "Characterisation of the Nucleotide and DNA Coeffictor Binding Sites of the Herpes Simplex Virus Type 1 (HSV-1) Encoded Helicase-Primase Complex and UL9 Origin Binding Protein", Biochemical and Biophysical Research Communications, vol. 199, No. 3, Mar. 30, 1994, pp. 1333-1340.
Koff, A. et al., "Herpes Simplex Virus Origin-Binding Protein (UL9) Loops and Distorts the Viral Replication Origin", Journal of Virology, vol. 65, No. 6, Jun. 1991, pp. 3284-3292.
Gustafsson, C. et al., "Herpes simplex virus DNA replication: A spacer sequence directs the ATP-dependent formation of a nucleoprotein complex at ori.sub.s ", Proc. Natl. Acad. Sci. USA, vol. 91, pp. 4629-4633, May 1994.
Hazuda, D. et al., "Characterization of Herpes Simplex Virus Origin Binding Protein Interaction with Ori.sub.s ", The Journal of Biological Chemistry, vol. 266, No. 36, Dec. 25, 1991, pp. 24621-24626.
Koff, A. et al., "Characterization of Major Recognition Sequences for Herpes Simplex Virus Type 1 Origin-Binding Protein", Journal of Virology, vol. 62, No. 11, Nov. 1988, pp. 4096-4103.
Hammarsten, O. et al., "Characterization of a binding site for the herpes simplex virus type 1 UL9 origin-binding protein within the UL9 gene", Journal of General Virology, vol. 77, pp. 969-976, (1996).
Martin, D.W. et al., "Analysis of the DNA-Binding Domain of the HSV-1 Origin-Binding Protein", Virology, vol. 198, pp. 71-80, 1994.
Arbuckle, M. et al., "A mutational analysis of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein", Journal of General Virology, vol. 74, pp. 1349-1355, (1993).
Stow, N., "Herpes simplex virus type 1 origin-dependent DNA replication in insect cells using recombinant baculoviruses", Journal of General Virology, vol. 73, pp. 313-321, (1992).
Wu, C. et al., "Identification of Herpes Simplex Virus Type 1 Genes Required for Origin-Dependent DNA Synthesis", Journal of Virology, vol. 62, No. 2, Feb. 1988, pp. 435-443.
Challberg, M., "A Method for identifying the viral genes required for herpesvirus DNA replication", Proc. Natl. Acad. Sci. USA, vol. 83, pp. 9094-9098, Dec. 1986.
Martinez, R. et al., "The Conserved Helicase Motifs of the Herpes Simplex Virus Type 1 Origin-Binding Protein UL9 Are Important for Function", Journal of Virology, vol. 66, No. 11, Nov. 1992, pp. 6735-6746.
Chen, D. et al., "Varicella-Zoster Virus Gene 51 Complements a Herpes Simplex Virus Type 1 UL9 Null Mutant", Journal of Virology, vol. 69, No. 7, Jul. 1995, pp. 4515-4518.
Inoue, N. et al., "Human Herpesvirus 6B Origin-Binding Protein: DNA-Binding Domain and Consensus Binding Sequence", Journal of Virology, vol. 69, No. 8, Aug. 1995, pp. 4619-4627.
Inoue, N. et al., "Alphaherpesvirus Origin-Binding Protein Homolog Encoded by Human Herpesvirus 6B, a Betaherpesvirus, Binds to Nucleotide Sequences That Are Similar to ori Regions of Alphaherpesviruses", Journal of Virology, vol. 68, No. 7, Jul. 1994, pp. 4126-4136.
Chen, D. et al., "Expression of the Varicella-Zoster Virus Origin-Binding Protein and Analysis of Its Site-Specific DNA-Binding Properties", Journal of Virology, vol. 68, No. 6, Jun. 1994, pp. 3841-3849.
Martin, D. et al., "Analysis of the Herpes Simplex Virus Type 1 Ori.sub.s Sequence: Mapping of Functional Domains", Journal of Virology, vol. 65, No. 8, Aug. 1991, pp. 4359-4369.
Lockshon, D. et al., "Sequence and Structural Requirements of a Herpes Simplex Viral DNA Replication Origin", Molecular and Cellular Biology, vol. 8, No. 10, Oct. 1988, pp. 4018-4027.
Hardwicke, M. et al., "Cloning and Characterization of Herpes Simplex Virus Type 1 oriL: Comparison of Replication and Protein-DNA Complex Formation by oriL and oriS", Journal of Virology, vol. 69, No. 3, Mar. 1995, pp. 1377-1388.
Wong, S. et al., "Elements in the Transcriptional Regulatory Region Flanking Herpes Simplex Virus Type 1 oriS Stimulate Origin Function", Journal of Virology, vol. 65, No. 5, May 1991, pp. 2601-2611.
Deb, S. et al., "A 67-Base-Pair Segment from the Ori-S Region of Herpes Simplex Virus Type 1 Encodes Origin Function", Journal of Virology, vol. 62, No. 7, pp. 2516-2519.
Klupp, B. et al., "The Virulence-Determining
Lee Sam S.-K.
Mosher Mary E.
Salimi Ali R.
Weitz David J.
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