Kojibiose phosphorylase obtainable from Thermoanaerobium brockii

Details Kojibiose phosphorylase obtainable from Thermoanaerobium brockii Kojibiose phosphorylase obtainable from Thermoanaerobium brockii

1997-11-07

1999-10-12

Weber, Jon P.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435194, C12P 1912, C12N 912

Patent

active

059654120

ABSTRACT:
A kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form .beta.-glucose and .beta.-D-glucose-1-phosphoric acid, forms kojibiose and an inorganic phosphoric acid from .beta.-D-glucose-1-phosphoric acid, and catalyzes the transfer reaction of glucosyl group to other saccharides using .beta.-D-glucose-1-phosphoric acid as a saccharide donor. The enzyme is obtainable from Thermoanaerobium brockii and obtainable by recombinant technology.

REFERENCES:
"Enzyme handbook", Asakura-Shoten Publisher, Tokyo, Japan (1982).
Sambrook et al., "Molecular cloning: a laboratory manual, 2nd ed.", Cold Spring Harbor Laboratory Press, USA (1989).
Hatt, H.D. ed. et al., "Catalogue of bacteria and phages, 18th ed.", American Type Culture Collection, USA (1992).
ATCC Catalog Number 35047, Nov. 1998.
ATCC Catalog Number 53556, Nov. 1998.
Lee et al., Int. J. Syst. Bact., 43(1), "Taxonomic Distinction of Saccharolytic Thermophilic Anaerobes . . . ", pp. 41-51, 1993.

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