Method and reagents for quantitative determination of reticulocy

Details Method and reagents for quantitative determination of reticulocy Method and reagents for quantitative determination of reticulocy

1980-08-15

1982-06-22

Turk, Arnold

Chemistry: physical processes

Physical processes

Crystallization

250461B, 252408, G01N 2164, G01N 3352

Patent

active

043360297

ABSTRACT:
Methods and compositions for quantitating reticulocytes and platelets by fluorescence in a flow cytometer. The composition comprises an aqueous solution of the dye acridine orange, citrate ion, and para-formaldehyde at a pH of about 7.4 and an isotonic osmolality. The concentrations of the various ingredients are selected to maximized dye uptake by the reticulocytes and platelets to allow for the first time the fluorescent quantitation of these cells in whole blood using a flow cytometer.

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patent: 3684377 (1972-08-01), Adams et al.
patent: 3864571 (1975-02-01), Stillman et al.
patent: 3883247 (1975-05-01), Adams
patent: 3916205 (1975-10-01), Kleinerman
patent: 3997656 (1976-12-01), Wertlake et al.
patent: 4027971 (1977-06-01), Kolman et al.
patent: 4146604 (1979-03-01), Kleinerman

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