Metachromatic dye sorption means for differential determination

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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424 71, 436 63, G01N 130, G01N 3349, G01N 3352

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active

046158782

ABSTRACT:
Differentiation, identification and enumeration of sub-populations of lymphocytes including B-cells and T-cells can now be further discriminated into their sub-populations by means of basic orange 21 as a supravital stain to provide distinctive morphologic differences between B-cells, T-suppressor cells, T-helper cells and natural killer (NK) cells without resorting to the use of individual monoclonal antibodies for differentiation of each of the above.
The differentiation, identification and enumeration of the foregoing cells can be accomplished by means of both white light absorbance and fluorescence measurements, singly or in sequential combinations.

REFERENCES:
patent: 2126852 (1938-08-01), Wolff
patent: 3617185 (1971-11-01), Drautz
patent: 4146604 (1979-03-01), Kleinerman
patent: 4390518 (1983-06-01), Fischer
patent: 4400370 (1983-08-01), Kass
patent: 4492752 (1985-01-01), Hoffman
patent: 4500509 (1985-02-01), Kass
patent: 4581223 (1986-04-01), Kass
Lanier et al. (1983), J. of Immunology 131(4): 1789-1796.

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