Methods for detecting nucleic acid sequences

Details Methods for detecting nucleic acid sequences Methods for detecting nucleic acid sequences

1988-04-29

1991-04-30

Spiegel, Jack

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 19, 435 21, 436501, 536 26, 536 27, 536 28, 935 77, 935 78, C12Q 168, C12Q 142, G01N 33566, C07H 1512

Patent

active

050117696

ABSTRACT:
This invention provides a method for detecting a target nucleic acid which comprises forming a reaction mixture which includes the target nucleic acid and an amount of a complementary single-stranded nucleic acid probe which is greater than the target molecule, under conditions which allow the probe and the target nucleic acid to hybridize to each other and form a double stranded target-probe complex, nicking the hybridized probe at least once within a predetermined sequence so as to form at least two probe fragments hybridized to the target nucleic acid, resulting in the probe fragments to become single-stranded and allowing the target nucleic acid to become hybridized to another probe; and identifying probe fragments, thereby detecting the target nucleic acid. This invention also provides a method for detecting a target nucleic acid.

REFERENCES:
patent: 4795701 (1989-01-01), Vary
patent: 4820630 (1989-04-01), Taub

Affiliated with

Also associated with

Rating

Methods for detecting nucleic acid sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for detecting nucleic acid sequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for detecting nucleic acid sequences will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-640067