Method for soybean transformation and regeneration

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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800205, 800DIG26, 43524049, C12N 1582

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active

054160111

ABSTRACT:
A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.

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