Method for rapid toxicity testing of a liquid sample

Details Method for rapid toxicity testing of a liquid sample Method for rapid toxicity testing of a liquid sample

1993-11-15

1995-05-16

Beisner, William H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 721, 435 32, C12Q 102, C12Q 118

Patent

active

054160057

ABSTRACT:
A toxicity testing assay wherein a test sample is prepared with electrochromic dye such as Di-4-ANEPPS and a living organism of the cladoceran order, Daphnia Spp. or other test organisms, and the sample is irradiated with alternately blue and yellow light to excite fluorescence. The successive groups of fluorescent emissions are then viewed at 90.degree. by a photomultiplier which develops equivalent alternate count outputs, and the count output is amplified and processed to develop data indicating (1) membrane potential of cells of the living organisms and (2) the total dye fluorescence, which combined effects provides indication of deleterious effects to the organisms.

REFERENCES:
patent: 5094944 (1992-03-01), Hayes
Montana, V., Dual-Wavelength Ratiometric Fluorescence . . . Biochemistry, (1989), 28 pp. 4536-4539.
Sanchez, P., Toxicity Assessment of Industrial Effluents . . . Toxicity Assessment: An International Journal, vol. 3, 55-80 (1988).

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