Method of detecting elastase IV

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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4353201, 435325, 536 231, 536 232, 536 235, 536 2431, C12Q 168, C12N 1563, C12N 1585, C07H 2104

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057389918

ABSTRACT:
The present invention provides nucleotide and amino acid sequences that identify and encode a human homolog of rat elastase IV (HEIV) expressed in pancreas tissue. The present invention also provides for antisense molecules to the nucleotide sequences which encode HEIV, hybridization probes or oligonucleotides for the detection of HEIV-encoding nucleotide sequences, and a diagnostic test based on HEIV-encoding nucleic acid molecules. The present invention further provides for genetically engineered host cells for the expression of HEIV, biologically active HEIV, antibodies capable for binding specifically to HEIV, and treatment methods comprising administration of compounds, such antibodies or inhibitors, capable of binding HEIV to alter its activity.

REFERENCES:
Ngo et al, Computational complexity, protein structure prediction, and the Levinthal paradox, The Protein Folding Problem and Tertiary Structure Prediction, pp. 433-440, 1994.
Gewirtz et al., Facilitating oligonucleotide delivery: helping antisense deliver on its promise, Proc.Natl. Acad. Sci., vol. 93, pp. 3161-3163, Apr. 1996.
James, Towards gene inhibition therapy: a review of progress and prospects in the field of antiviral antisense nucleic acids and ribozymes, Antiviral Chemistry and Chemotherapy, vol. 2(4), pp. 191-214, 1991.
Edwards, P.D. et al., "Synthetic Inhibitors of Elastase", Med. Res. Rev., Mar. 1994, 142(2) pp. 127-194 -- Abstract Only.
Gross, V. et al., "Inflammatory Mediators and Cytokins -- New Aspects of the Pathophysiology and Assessment of Severity of Acute Pancreatitis?", Hepatogastroenterology, Dec. 1993, 40(6) pp. 522-530 -- Abstract Only.
Kang, J. et al., "Identification of cDNSs Encoding Two Novel Rat Pancreatic Serine Proteases", Gene, 110 (2), 181-187 (1992) Abstract Only (EMBL accession X59014).

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