Analytical reagent particle with covalently-bound enzyme

Details Analytical reagent particle with covalently-bound enzyme Analytical reagent particle with covalently-bound enzyme

1996-10-16

1998-04-14

Knode, Marian C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 794, 435 795, 436518, 436527, G01N 3353

Patent

active

057389861

ABSTRACT:
The invention relates to a reagent consisting of at least one enzyme and at least one other substance, which are covalently or noncovalently bound to a particle, which is smaller than or is equal to 1000 Angstrom in diameter. The invention also relates to method and use of the reagent for determination or studies of a cell or a virus or another component in a sample. According to the invention, these determinations are made with for example some ELISA-technique (competitive, sandwich, etc.), employing the reagent preferrably in the form of a suspension in buffered water, instead of and in the same way as described for soluble enzyme conjugates. The substance can be an antibody, a lectin, avidin or an antigen, the enzyme can be for example peroxidase, alkaline phosphatase, and the particle can be an inorganic or an organic polymeric compound or a combination thereof, as, for example, tresyl-activated glycerylpropyl-silica.

REFERENCES:
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4552839 (1985-11-01), Gould et al.
Nilsson et al., Immobilization of Ligands with Organic Sulfonyl Chlorides, Methods in Enzymology 104:56-69, 1984.

Affiliated with

Also associated with

Rating

Analytical reagent particle with covalently-bound enzyme does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Analytical reagent particle with covalently-bound enzyme, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Analytical reagent particle with covalently-bound enzyme will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-633675