In vitro test to evaluate the inhibition potency of angiotensin

Details In vitro test to evaluate the inhibition potency of angiotensin In vitro test to evaluate the inhibition potency of angiotensin

1994-12-14

1997-07-29

Ketter, James S.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435370, C12Q 168

Patent

active

056520972

ABSTRACT:
The present invention relates to an in vitro test by recombinant DNA technology for the evaluation of the potency of various angiotensin II (AII) antagonists on the inhibition of expression of angiotensinogen (ANG) gene in vivo. A transformed human liver cell line capable of expressing human angiotensinogen-growth hormone, which includes at least one DNA construct coding for at least a 5'-flanking region of the ANG operatively linked upstream to human growth hormone gene. An in vitro test for the evaluation of the potency of various angiotensin II (AII) antagonists on the inhibition of expression of angiotensinogen (ANG) gene in vivo, which comprises administering AII antagonists to a transformed human liver cell line of claim 1 and monitoring the expression of human angiotensinogen-growth hormone.

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