Purification of hemoglobin and modified hemoglobin by affinity c

Details Purification of hemoglobin and modified hemoglobin by affinity c Purification of hemoglobin and modified hemoglobin by affinity c

1986-03-14

1990-05-15

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 530385, 530413, 530417, B01D 1508

Patent

active

049255746

ABSTRACT:
The invention disclosed relates to a method for the purification of hemoglobin by the technique of affinity chromatography. Contrary to current belief, the binding of oxygen and selected polyanions to hemoglobin is not mutually exclusive. The novel method comprises immobilizing a polyanion which specicically binds hemoglobin via its polyanion binding site, on a chromatographic gel and passing the hemoglobin containing solution or mixture through the gel. The hemoglobin is thus retained in the gel, while impurities are eluted from the gel. The novel method is also applicable to the separation of unmodified hemoglobin from a liquid reaction mixture containing modified and unmodified hemoglobin.

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