Plasmidless Lac strain of bacilus megaterium QM B1551

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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4351723, 4352525, C12N 120, C12N 1500

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active

050305743

ABSTRACT:
A plasmidless Lac mutant strain of Bacillus megaterium QM B1551 designated PV447 is provided which otherwise has substantially all of the characteristics of the parent stock.
B. megaterium PV447 has been deposited at the NRRL on June 19, 1987 and been given NRRL accession No. B-18233.

REFERENCES:
patent: 4190495 (1980-02-01), Curtiss, III
Stahl et al.; Chem. Abstr. 99: 100158v (1983).
Vary, P. S., Garbe, J. C., Franzen, M. A. and Frampton, E. W., 1982, MP13, A Generalized Transducing Bacteriophage for Bacillus megaterium, J. Bacteriol. 149:1112-1119.
Garbe, J. C. and Vary, P. S., 1981, Bacteriophage MP13 Transduction of Bacillus megaterium OM B1551, pp. 83-87, In: Sporulation and Germination, H. S. Levinson, A. L. Sonenshein and D. J. Tripper (eds.), American Society for Microbiology, Washington, D.C.
Callahan, J. P., Crawford, I. P., Hess, G. F. and Vary, P. S., 1983, Cotransductional Mapping of the trp-his region of Bacillus megaterium, J. Bacteriol. 154:1455-1458.
Garbe, J. C., Hess, G. F., Franzen, M. A. and Vary, P. S., 1984, Genetics of Leucine Biosynthesis in Bacillus megaterium, J. Bacteriol. 157:454-459).
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Vorobjeva, I. P., Khmel, I. A. and Alfoldi, L., 1980, Transformation of Bacillus megaterium Protoplasts by plasmid DNA, FEMS Microbiology Letters, 7:261-263.
Kieselburg, M. K., Weikert, M. and Vary, P. S., Analysis of Resident and Transformant Plasmids in Bacillus megaterium, Bio/technology, 2:254-259.
Bohall, N. A., and Vary, P. S., Transposition of Tn917 in Bacillus megaterium, J. Bacteriol. 167:716-718.
Von Tersch, M. A. and Carlton, B. C., 1984, Molecular Cloning of Structural and Immunity Genes for Megacins A-216 and A-19213 in B. megaterium, J. Bacteriol. 160:854-859.
Weiland, Katherine Lee, M. S. Thesis, Department of Biological Sciences, Northern Illinois University, Plasmid Analysis of Megacin Negative Strains of Bacillus megaterium QM B1551.

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