Immobilization of proteins on polymeric supports

Details Immobilization of proteins on polymeric supports Immobilization of proteins on polymeric supports

1982-09-30

1985-09-03

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

260112R, 435177, 435181, C12N 1108, C12N 1102, C12N 1106, C07G 700

Patent

active

045392943

ABSTRACT:
A protein is immobilized by a simple and effective method on a porous polymeric support by a first soaking in a dilute long-chain cationic solution and a second soaking in a dilute aqueous protein solution. The long chain cationic is preferably a nitrogen compound such as a diamine having at least one alkyl or alkenyl group containing at least eight carbon atoms. A preferred diamine is N-coco-1,3-diamino-propane. The protein may be an enzyme or hormone. A preferred enzyme is catalase or a two enzyme system of catalase and glucose oxidase.

REFERENCES:
patent: 3841969 (1974-10-01), Emery et al.
patent: 4043869 (1977-08-01), Barker et al.
patent: 4072566 (1978-02-01), Lynn
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, (pp. 145-147).

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