Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1985-02-25
1988-02-23
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
356 39, 356 73, 435 7, 435 29, 435 34, 436 63, 436 72, 436501, 436519, 436548, 436800, 436827, 935110, C12Q 104, C12Q 168, G01N 33554, G01N 33577
Patent
active
047270209
ABSTRACT:
A method for identifying and enumerating cells of a subclass of blood cells in relation to cells of another subclass of blood cells is provided. In the method a first subclass of blood cells is selectively tagged by incubating an aliquot of a blood sample with a first tagging agent. A second subclass of blood cells is selectively tagged by incubating the aliquot with a second tagging agent. The aliquot is then passed, without lysing of any subclasses of blood cells which are not of interest, substantially a cell at a time through an area of optical stimulation for the tagging agents. Light emitted by the tagging agents is detected, the detection being limited by gating to a threshold value related to a predetermined intensity of light by one of the tagging agents. Cells of the subclass are differentiated based on occurrence of emitted light from the tagging agents.
REFERENCES:
patent: 3883247 (1975-05-01), Adams
H. M. Shapiro, Cytometry, 3, 227-243, 1983.
M. R. Melino et al., Journ. Immunol. 130, 1843-1847, 1983.
R. A. Miller et al., Journ. Immunol. 133, 3408-3414, 1984.
Becton Dickinson and Company
Hallenbeck Robert M.
Marantz Sidney
Saunders David A.
LandOfFree
Method for analysis of subpopulations of blood cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for analysis of subpopulations of blood cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for analysis of subpopulations of blood cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-602591