Process and apparatus for conducting electrophoresis and transfe

Details Process and apparatus for conducting electrophoresis and transfe Process and apparatus for conducting electrophoresis and transfe

1986-02-04

1988-02-23

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2728, G01N 2726

Patent

active

047268891

ABSTRACT:
An apparatus for carrying out horizontal gel electrophoresis for separation and subsequent vacuum-assisted transportation of macromolecules to a support membrane to facilitate detection. The entire procedure is conducted in one cartridge. A method for conducting electrophoresis and subsequent vacuum-assisted transfer using the apparatus of the present invention is also disclosed.

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M. Peferoen et al, "Vacuum-Blotting: a New Simple and Efficient Transfer of Proteins from Sodium Dodecyl sulfate-Polyacrylamide Gels to Nitrocellulose", Febs. Letters, 1982, vol. 145, No. 2, pp. 369-371.
Zaitsev, I. Z. et al., "Vacuum Transfer at DNA to Filters for Detecting Inter-Individual Polymorphism by the Southern Blotting Hybridization Method", Byull. Eksp. Biol. Med., 1983, 96(10), p. 846, (Abstract only).
E. M. Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis", Journal of Molecular Biology, (1975), 98, 503-517.
M. Bittner et al., "Electrophoretic Transfer of Proteins and Nucleic Acids from Slab Gels to Diazobenzyloxymethyl Cellulose or Nitrocellulose Sheets", Analytical Biochemistry 102, 459-471, (1980).
Bethesda Research Laboratories, Inc., "Catalogue & Reference Guide", Aug. 1983, p. 51.

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