System and apparatus for plant tissue culture propagation

Plant husbandry – Process

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47 17, A01G 3100

Patent

active

044635222

ABSTRACT:
This invention relates to a method for rapid plant propagation through tissue culture, which method comprises commutating a sterile explant to provide a multiplicity of explant particles each capable of reproducing the parent plant, suspending the reproducing explant particles in a fluid medium having a viscosity sufficiently high to prevent rapid settling of the explant particles, aseptically dispensing said suspended explant particles at spaced intervals into a presterilized growth supporting bed comprising a natural or synthetic soil mix or a gelled growth supporting substrate, such as agar; aseptically enclosing the resultant reproducing explant particle inoculated bed in a light transparent, moisture retaining plant growth space providing envelope, allowing propagation of rooted plants from said explant particles, and then hardening off the resultant plants by providing an opening in said envelope of a size sufficient to allow loss of humidity from the envelope at a rate sufficiently slow to prevent irreversible drying of leaves or a substantial degree of shock. The invention also relates to the apparatus employed in the process.

REFERENCES:
Clonal Propagation of Ginger . . . , Hosoki et al., Hortscience, vol. 12(5), Oct. 1977, pp. 451-452.

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